No, fuck off.
A meme of unknown origin. It's very likely to be a counter of the famous "Do you have...um..." meme.
The "Do you have...um..." meme usually depicts a person asking a question, but shying away from the very last part, thus leaving his question somewhat vague. More description can be added into the sentence to further clarify what his exact goal is.
It is implied that the person is either completely oblivious to some extremely obvious thing, or just faking it for shits and giggles. Therefore, the correct way of responding is tell him to fuck off, instead of actually helping him.
e.g.
A: Do you have...um...that game where you can play as a....you know...yellow jumpsuit wearing girl with some nice ass...you know...she wears a goggle and can teleport....?
B: No, fuck off.
